WebTo investigate the effect of PHGH-modified starch on E. coli growth, an atomic force microscope (AFM) using a Nanoscope IIIa (Veeco Instruments) was employed. Initially, fresh E. coli from the LB broth were separated from the supernatant by centrifuging the bacterial suspension (108 CFU/mL) at 5000 rpm for 1 min.
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WebFeb 1, 2024 · Polyhexamethylene guanidine hydrochloride (PHGH), was selected because of its known ability to inhibit Gram-positive and Gram-negative bacteria (Zhou, Zheng, & Zhong, 2011). PHGH is a cationic antimicrobial oligomer that is typically synthesized by a polycondensation reaction between hexamethylene diamine and guanidine hydrochloride. WebMar 2, 2008 · Polyhexamethylene guanidine hydrochloride (PHGH) was synthesized according to the procedures described elsewhere (Zhang et al. 1999 ). Luria Bertani (LB) broth, LB agar and phosphate buffered saline (PBS, pH = 7.4) were used for the cultivation of bacteria. Distilled deionized water was used in all the experiments. WebSep 15, 2014 · As we mentioned above, PHGH molecule has longer linear molecular chain and larger molecular size compared with HDA molecule, and thus would lead to a lower chance for PHGH to pass through the water/oil interfacial zone and further affect the cross-linking reaction with TMC in organic phase. list of applications on computer